Process of manufacturing glandular extractive product



UNITED STATES PATENT OFFICE.

.enrnonsouon'ron' CLOVER, or DETROIT, MICHIGAN, ASSIGNOR 'ro PARKE Da i &

COMPANY, or DETROIT, MICHIGAN, A oonrona'rxon or moment.

PROCESS OF MANUFACTURING GLANDUI'AR EXTRACTIVE PRODUCT.

No Drawing.

Toall whom it may concern/.1

Be it known that I, ALrHoNgso MORTON C OVER, a citizen of the United States, residing atD'etroit, in the county of WVayne and State of Michigan, have invented certain new and useful Improvements in Processes of Manufacturing Glandular Extractive Product, of which the following is ,a speoification.

My invention consists of a novel process of manufacturing in the form of a solid the active principle of the in-fundibular portion of the pituitary gland.

A water extract of the therapeutically active substance contained in the infundibular or osterior lobe of the pituitary gland is at t e present time made and sold by di erprinciple of suprarenal glands) but is much more prolonged. Y

The above extract has been found to be very unstable-that is, it decomposes on standing and becomes less and less effective.

. It is, therefore, desirable to isolate the active substance ina solid, friable or powdered state and in a soluble condition, in which condition it can be preserved indefinitely, and is immediately available for use by dissolvin it when needed.

n practising my process I start w1th a water extract of the said infundibular lobe of the said gland and precipitate out of it the active prmciple by saturating it with a suitable salt. As an example of my process, the following may be given:

A water extract of the infundibula-r lobe of the pituitary gland may be obtained in any well-known or suitable way, for exam= ple, as by digesting the macerated material with hot acidulated water and removing the insoluble residue by filtration. This solution I saturate'with a suitable water soluble salt preferably df the alkali or alkaline earth Specification of 'Lettei's Patent.

Patented Apr. 5, 1921.

Application filed March 3, 1913. Serial No. 751,957.

metals which should be freely soluble in water, non-toxlc and neutral or slightly acid 1n reaction toward litmus such as sodium chlorid, sodium sulfate, magnesium sulfate, ammomum sulfate, etc., which precipitates the active principle from the solution. By. preference I use sodium chlorid. The preclpltate is filtered, dried in the air and powdered. The resulting product, when sod um chlorid is used, contains some sodlum chlor d, but in commercial practice it is not necessary to remove this, as its presence is not objectionable; but it may, of course, beremoved, if desired. By alkaline salt, as used in the claims, I include salts of the alkali metals and salts of the alkaline earth metals.

The process of removing the active princlple by precipitation with sodium chlorid 1s almost, or practically, complete, provided the solutlon is sufficiently strong (say three parts of the solution representing one part of the infundibular portion of the gland), as the precipitated product when redissolved in water is nearly as potent therapeutically as the original solution. The precipitated product constitutes only a small portion of the extractive matter contained in the original solution, showing that a separation has been made, and that the active substance has been isolated from the inert and undesirable constituents of the solution.

The product is a white or nearly white I fiufl'y powder which shows all the therapeutical propertles of pituitary extract.

That the product is a highly potent one therapeutically, that the inert and undesirable matter contained in the extract has been mostly eliminated, and that it is at least a close approximation to, if not the chemically pure active principle of the pituitary gland, may be judged from the fact that a fraction of a milligram of the active substance, exclusive of the sodium chlo-rid contained in the same as described above, is an active dose 1 on the occasion of human child-birth.

A solution of the product is precipitated by many of the so-called alkaloidal precipitants, such as phospho-tungstic acid, picric acid, mercuric potassium iodid, platinic 105 present. It gives the biuret reaction. 'It is 110 soluble in Water, preferably acidulated, and glycerin of all strengths; but is insoluble in strong alcohol, acetone, etc. On the other hand it gives no precipitate with mercuric bichlorid, ferric chlorid, and lead acetate when an excess of these reagents is not used so as to produce a salting out effect. It gives no precipitate with uranium acetate.

What I claim and desire to secure by Letters Patent of the United States is 1. The process of obtaining the active principle of the infundibular lobe of the pituitary gland in the form of a solid which consists in making a water extract of said lobe, and precipitating the active material therefrom by saturating it with a freely soluble, non-toxic, alkaline metal salt having a neutral or slightly acid reaction.

2. The process of obtaining the active principle of the infundibular lobe of the pituitary gland in the form of a solid which consists in making a water extractof said lobe,

by treating the lobe with acidulated water, and precipitating the active miterial therefrom by saturating it with sodium chlorid.

3. The process of obtaining the active principle of the infundibular lobe of the pituitary gland in the form of a. solid which consists in making a water extract of .the gland by treating With acidulated water, heating and removing the insoluble residue formed, and precipitating the active material from the remaining solution by saturating it with a freely soluble, non-toxic, alkaline metal salt having a neutral'or slightly acid reaction.

4. The process of obtaining the active principle of the infundibular lobe of the pituitary gland in the form of a solid which consists in makin a water extract of the gland with hot acidulated water, removing the insoluble residue, and precipitating the active material from the remaining solution by saturating it with a freely soluble, nontoxic, alkaline metal salt having a neutral or sli htly acid reaction.

5. he process of obtaining the active principle of the infundibular lobe of the pituitary gland in the form of a solid which consists in making a water extract of the gland with hot acidulated water, removing the insoluble residue, and precipitating the active material from the remaining solution by saturating it with sodium chlorid.

In testimony. whereof I have hereunto signed my name in the presence of two subscribing witnesses.

ALPHONSO MORTON CLOVER.

Witnesses J. EDGAR BULL, M. E. MCNINCH. 

